Johnson, Stuart, Dr

Dr Stuart Johnson

School of Biosciences

Lecturer

s.johnson@sheffield.ac.uk
+44 114 222 3692

Alfred Denny Building

Full contact details

Dr Stuart Johnson
School of Biosciences
Alfred Denny Building
Western Bank
91̽��
S10 2TN

Profile

2011-present: Royal Society University Research Fellow, University of 91̽��, UK.
2009-2011: RNID Postdoctoral Fellow, University of 91̽��, UK.
2006-2009: Wellcome Trust Postdoctoral Fellow, University of 91̽��, UK.
2003-2005: MRC Postdoctoral Fellow, University of Sussex, UK.
1998-2003: D.Phil, University of Sussex, UK.
1995-1998: BSc Hons Biological Sciences (Animal Physiology), University of Leicester, UK.

Research interests

Signalling characteristics of cochlear hair cells.

Auditory neuroscience, sensory coding, synaptic transmission

Mammalian cochlear inner hair cells (IHCs) are the primary sensory cells of the auditory pathway. Their job is to convert sound vibrations into an electrical signal that can be interpreted by the brain. As such, it is vital that the information encoded by IHCs is accurately preserved at this initial stage. One of the major causes of deafness/hearing loss is associated with IHCs losing their ability to function normally.

The aim of my research is to find out how IHCs are able to accurately encode sounds over a wide frequency and intensity range and how the information is processed on its way to the brain. Knowledge of how the ear processes sound will be informative to develop improved hearing aids, including cochlear implants. An additional aspect of my research applies directly to define how stem cells are able to replace damaged nerve fibres in order to restore hearing (in collaboration with Prof Marcelo Rivolta).

In order to achieve this I will study IHCs in the isolated cochlea using a combination of electrophysiological, cell imaging and molecular biological techniques.

Publications

Show: Featured publications All publications

Featured publications

Journal articles

Johnson SL, Olt J, Cho S, von Gersdorff H & Marcotti W (2017) . Journal of Neuroscience, 37(9), 2471-2484.
Johnson SL, Ceriani F, Houston O, Polishchuk R, Polishchuk E, Crispino G, Zorzi V, Mammano F & Marcotti W (2017) . Journal of Neuroscience, 37(2), 258-268.
Corns LF, Johnson SL, Kros CJ & Marcotti W (2014) . Proceedings of the National Academy of Sciences, 111(41), 14918-14923.
Furness DN, Johnson SL, Manor U, Rüttiger L, Tocchetti A, Offenhauser N, Olt J, Goodyear RJ, Vijayakumar S, Dai Y , Hackney CM et al (2013) . Proc Natl Acad Sci U S A, 110(34), 13898-13903.
Johnson SL, Kuhn S, Franz C, Ingham N, Furness DN, Knipper M, Steel KP, Adelman JP, Holley MC & Marcotti W (2013) . Proc Natl Acad Sci U S A, 110(21), 8720-8725.
Chen W, Jongkamonwiwat N, Abbas L, Eshtan SJ, Johnson SL, Kuhn S, Milo M, Thurlow JK, Andrews PW, Marcotti W , Moore HD et al (2012) . Nature, 490(7419), 278-282.
Johnson SL, Kennedy HJ, Holley MC, Fettiplace R & Marcotti W (2012) . J Neurosci, 32(31), 10479-10483.
Johnson SL, Beurg M, Marcotti W & Fettiplace R (2011) . Neuron, 70(6), 1143-1154.
Johnson SL, Eckrich T, Kuhn S, Zampini V, Franz C, Ranatunga KM, Roberts TP, Masetto S, Knipper M, Kros CJ & Marcotti W (2011) . Nature Neuroscience, 14(6), 711-717.
Johnson SL, Franz C, Kuhn S, Furness DN, Rüttiger L, Münkner S, Rivolta MN, Seward EP, Herschman HR, Engel J , Knipper M et al (2010) . Nat Neurosci, 13(1), 45-52.
Johnson SL () . eLife, 4.

All publications

Journal articles

Carlton AJ, Jeng J, Grandi FC, De Faveri F, Ceriani F, De Tomasi L, Underhill A, Johnson SL, Legan KP, Kros CJ , Richardson GP et al (2023) . The EMBO Journal.
Jeng J-Y, Carlton AJ, Goodyear RJ, Chinowsky C, Ceriani F, Johnson SL, Sung T-C, Dayn Y, Richardson GP, Bowl MR , Brown SDM et al (2022) . Molecular Therapy - Methods & Clinical Development, 26, 355-370.
Spaiardi P, Marcotti W, Masetto S & Johnson SL (2022) . Frontiers in Cellular Neuroscience, 16.
Lorente-Cánovas B, Eckrich S, Lewis MA, Johnson SL, Marcotti W & Steel KP (2022) . PLoS ONE, 17(3).
Manca M, Yen P, Spaiardi P, Russo G, Giunta R, Johnson SL, Marcotti W & Masetto S (2021) . Frontiers in Neuroscience, 15.
Jeng J, Harasztosi C, Carlton AJ, Corns LF, Marchetta P, Johnson SL, Goodyear RJ, Legan KP, Rüttiger L, Richardson GP & Marcotti W (2021) . The Journal of Physiology.
Jeng J, Carlton AJ, Johnson SL, Brown SDM, Holley MC, Bowl MR & Marcotti W (2021) . The Journal of Physiology, 599(1), 269-287.
Jeng J, Ceriani F, Olt J, Brown SDM, Holley MC, Bowl MR, Johnson SL & Marcotti W (2020) . The Journal of Physiology.
Spaiardi P, Marcotti W, Masetto S & Johnson SL (2020) . Physiological Reports, 8(14).
Jeng J, Johnson SL, Carlton AJ, DeTomasi L, Goodyear R, DeFaveri F, Furness DN, Wells S, Brown SDM, Holley MC , Richardson GP et al (2020) . The Journal of Physiology.
Spaiardi P, Tavazzani E, Manca M, Russo G, Prigioni I, Biella G, Giunta R, Johnson SL, Marcotti W & Masetto S (2020) . Neuroscience, 426, 69-86.
Jeng J, Ceriani F, Hendry A, Johnson SL, Yen P, Simmons DD, Kros CJ & Marcotti W (2020) . The Journal of Physiology, 598(1), 151-170.
Dunbar LA, Patni P, Aguilar C, Mburu P, Corns L, Wells HRR, Delmaghani S, Parker A, Johnson S, Williams D , Esapa CT et al (2019) . EMBO Molecular Medicine, 11(9).
Bardhan T, Jeng JY, Waldmann M, Ceriani F, Johnson SL, Olt J, Rüttiger L, Marcotti W & Holley MC (2019) . The Journal of Physiology.
Ceriani F, Hendry A, Jeng JY, Johnson SL, Stephani F, Olt J, Holley MC, Mammano F, Engel J, Kros CJ , Simmons DD et al (2019) . The EMBO Journal, 38(9), e99839-e99839.
Johnson SL, Safieddine S, Mustapha M & Marcotti W (2019) . Cold Spring Harbor Perspectives in Medicine, 9(12), a033175-a033175.
Chessum L, Matern MS, Kelly MC, Johnson SL, Ogawa Y, Milon B, McMurray M, Driver EC, Parker A, Song Y , Codner G et al (2018) . Nature, 563(7733), 696-700.
Corns LF, Johnson SL, Roberts T, Ranatunga KM, Hendry A, Ceriani F, Safieddine S, Steel KP, Forge A, Petit C , Furness DN et al (2018) . Nature Communications, 9.
(2017) . The Journal of Physiology, 595(11), 3677-3678.
Johnson SL, Olt J, Cho S, von Gersdorff H & Marcotti W (2017) . Journal of Neuroscience, 37(9), 2471-2484.
Johnson SL, Ceriani F, Houston O, Polishchuk R, Polishchuk E, Crispino G, Zorzi V, Mammano F & Marcotti W (2017) . Journal of Neuroscience, 37(2), 258-268.
Buniello A, Ingham NJ, Lewis MA, Huma AC, Martinez‐Vega R, Varela‐Nieto I, Vizcay‐Barrena G, Fleck RA, Houston O, Bardhan T , Johnson SL et al (2016) . EMBO Molecular Medicine, 8(3), 191-207.
Corns LF, Johnson SL, Kros CJ & Marcotti W (2016) . Journal of Neuroscience, 36(2), 336-349.
Carrott L, Bowl MR, Aguilar C, Johnson SL, Chessum L, West M, Morse S, Dorning J, Smart E, Hardisty-Hughes R , Ball G et al (2016) . Journal of Neuroscience, 36(1), 222-234.
Magistretti J, Spaiardi P, Johnson SL & Masetto S (2015) . FRONTIERS IN CELLULAR NEUROSCIENCE, 9.
Taylor R, Bullen A, Johnson SL, Grimm-Gunter E-M, Rivero F, Marcotti W, Forge A & Daudet N (2015) . Human Molecular Genetics, 24(1), 37-49.
Corns LF, Johnson SL, Kros CJ & Marcotti W (2014) . Proceedings of the National Academy of Sciences, 111(41), 14918-14923.
Olt J, Johnson SL & Marcotti W (2014) . J Physiol, 592(10), 2041-2058.
Chen J, Johnson SL, Lewis MA, Hilton JM, Huma A, Marcotti W & Steel KP (2014) . Eur J Neurosci, 39(5), 744-756.
Olt J, Mburu P, Johnson SL, Parker A, Kuhn S, Bowl M, Marcotti W & Brown SDM (2014) . PLoS One, 9(1), e87331.
Johnson SL, Wedemeyer C, Vetter DE, Adachi R, Holley MC, Elgoyhen AB & Marcotti W (2013) . Open Biol, 3(11).
Furness DN, Johnson SL, Manor U, Rüttiger L, Tocchetti A, Offenhauser N, Olt J, Goodyear RJ, Vijayakumar S, Dai Y , Hackney CM et al (2013) . Proc Natl Acad Sci U S A, 110(34), 13898-13903.
Zampini V, Johnson SL, Franz C, Knipper M, Holley MC, Magistretti J, Masetto S & Marcotti W (2013) . J Physiol, 591(16), 3811-3820.
Johnson SL, Kuhn S, Franz C, Ingham N, Furness DN, Knipper M, Steel KP, Adelman JP, Holley MC & Marcotti W (2013) . Proc Natl Acad Sci U S A, 110(21), 8720-8725.
Chen W, Jongkamonwiwat N, Abbas L, Eshtan SJ, Johnson SL, Kuhn S, Milo M, Thurlow JK, Andrews PW, Marcotti W , Moore HD et al (2012) . Nature, 490(7419), 278-282.
Eckrich T, Varakina K, Johnson SL, Franz C, Singer W, Kuhn S, Knipper M, Holley MC & Marcotti W (2012) . PLoS One, 7(9), e45732.
Johnson SL, Kennedy HJ, Holley MC, Fettiplace R & Marcotti W (2012) . J Neurosci, 32(31), 10479-10483.
Zuccotti A, Kuhn S, Johnson SL, Franz C, Singer W, Hecker D, Geisler H-S, Köpschall I, Rohbock K, Gutsche K , Dlugaiczyk J et al (2012) . J Neurosci, 32(25), 8545-8553.
Johnson SL, Beurg M, Marcotti W & Fettiplace R (2011) . Neuron, 70(6), 1143-1154.
Johnson SL, Eckrich T, Kuhn S, Zampini V, Franz C, Ranatunga KM, Roberts TP, Masetto S, Knipper M, Kros CJ & Marcotti W (2011) . Nature Neuroscience, 14(6), 711-717.
Zampini V, Rüttiger L, Johnson SL, Franz C, Furness DN, Waldhaus J, Xiong H, Hackney CM, Holley MC, Offenhauser N , Di Fiore PP et al (2011) . PLoS Biol, 9(4), e1001048.
Kuhn S, Johnson SL, Furness DN, Chen J, Ingham N, Hilton JM, Steffes G, Lewis MA, Zampini V, Hackney CM , Masetto S et al (2011) . Proc Natl Acad Sci U S A, 108(6), 2355-2360.
Zampini V, Johnson SL, Franz C, Lawrence ND, Münkner S, Engel J, Knipper M, Magistretti J, Masetto S & Marcotti W (2010) . J Physiol, 588(Pt 1), 187-199.
Johnson SL, Franz C, Kuhn S, Furness DN, Rüttiger L, Münkner S, Rivolta MN, Seward EP, Herschman HR, Engel J , Knipper M et al (2010) . Nat Neurosci, 13(1), 45-52.
Chen W, Johnson SL, Marcotti W, Andrews PW, Moore HD & Rivolta MN (2009) . Stem Cells, 27(5), 1196-1204.
Johnson SL, Franz C, Knipper M & Marcotti W (2009) . J Physiol, 587(Pt 8), 1715-1726.
Roux I, Hosie S, Johnson SL, Bahloul A, Cayet N, Nouaille S, Kros CJ, Petit C & Safieddine S (2009) . Human Molecular Genetics, 18(23), 4615-4628.
Johnson SL, Forge A, Knipper M, Münkner S & Marcotti W (2008) . J Neurosci, 28(30), 7670-7678.
Johnson SL & Marcotti W (2008) . J Physiol, 586(4), 1029-1042.
Johnson SL, Adelman JP & Marcotti W (2007) . J Physiol, 583(Pt 2), 631-646.
Marcotti W, Erven A, Johnson SL, Steel KP & Kros CJ (2006) . J Physiol, 574(Pt 3), 677-698.
Johnson SL, Marcotti W & Kros CJ (2005) . J Physiol, 563(Pt 1), 177-191.
Marcotti W, Johnson SL & Kros CJ (2004) . J Physiol, 560(Pt 3), 691-708.
Marcotti W, Johnson SL & Kros CJ (2004) . J Physiol, 557(Pt 2), 613-633.
Marcotti W, Johnson SL, Rusch A & Kros CJ (2003) . J Physiol, 552(Pt 3), 743-761.
Marcotti W, Johnson SL, Holley MC & Kros CJ (2003) . J Physiol, 548(Pt 2), 383-400.
Johnson SL, Thomas MV & Kros CJ (2002) . Pflugers Arch, 443(4), 653-663.
Conrad LJ, Grandi FC, Carlton AJ, Jeng J, de Tomasi L, Zarecki P, Marcotti W, Johnson SL & Mustapha M () . The Journal of Physiology.
Ceriani F, Johnson SL, Sedlacek M, Hendry A, Kachar B, Marcotti W & Mammano F () .
Frith TJR, Granata I, Stout E, Wind M, Thompson O, Neumann K, Stavish D, Heath PR, Hackland JOS, Anastassiadis K , Gouti M et al () .
Ceriani F, Hendry A, Jeng J-Y, Johnson SL, Olt J, Holley MC, Mammano F, Kros CJ, Simmons DD & Marcotti W () .
Zampini V, Johnson SL, Franz C, Knipper M, Holley MC, Magistretti J, Russo G, Marcotti W & Masetto S () . PLoS ONE, 9(11), e113750-e113750.
Johnson SL () . eLife, 4.

Chapters

Indzhykulian AA, Johnson SL & Géléoc GSG (2022) , Neuromethods (pp. 221-264). Springer US
Corns LF, Bardhan T, Houston O, Olt J, Holley MC, Masetto S, Johnson SL & Marcotti W (2014) , Development of Auditory and Vestibular Systems: Fourth Edition (pp. 155-188).
Corns LF, Bardhan T, Houston O, Olt J, Holley MC, Masetto S, Johnson SL & Marcotti W (2014) , Development of Auditory and Vestibular Systems (pp. 155-188). Elsevier
Alexander EE, Altieri SC, Avraham KB, Bardhan T, Baxendale S, Beraneck M, Bhonker Y, Cardon G, Chen P, Clause A , Contreras J et al (2014) , Development of Auditory and Vestibular Systems (pp. xi-xiii). Elsevier

Research group

Collaborations

Henrique von Gersdorff (Vollum Institute, USA)
Robert Fettiplace FRS (Wisconsin, USA)
Marcelo Rivolta (91̽��)

Grants

The Royal Society
Action on Hearing Loss

Teaching activities

Undergraduate and postgraduate taught modules

Undergraduate:

BMS248 Neural Circuits, Behaviour & Memory
BMS335 Sensory Neuroscience
Level 3 Practical and Dissertation Modules

Masters (MSc)

BMS6335 Sensory Neuroscience

Professional activities and memberships

Invited seminars

Sandbjerg Meeting on Membrane Transport, Denmark (2015)

Awards

Royal Society University Research Fellowship (2011)